FAQ

1. Why are my replicates not being meaned properly?

The most frequent reason for this comes from the numbering of the replicates. Indeed, for each group of same condition+time (example: Wild Type T0) the replicates must be numbered starting from 1 untill the last (example: Wild Type T0 1, Wild Type T0 2, Wild Type T0 3, etc…)

2. There was a problem during the experiment for one of my samples. How do I tell Isoplot not to take it into account?

The easiest way to remove any samples from the dataset is not to modify the dataset itself, but rather to remove the corresponding line from the template file.

3. Sometimes the generated directory for my plots has multiple sub-directories with the same name. Why is this?

This happens when we generate multiple times the directory by using the same name twice in a row (in the case of an error for example on the first try, or a mistake when selecting data to be plotted). A quick fix is to restart the Kernel or change the name of the directory to be created before generating the plots.

**4. I am trying to create barplots with meaned data (so with error bars) for isotopologue_fractions, but some of my bars seem abherrent. They have error bars that go until under 0 and the total of the fractions is not equal to 1. Why is this?

This happens when data is missing for only some replicates of a given sample condition & time. An ulterior version of Isoplot will fix this by not using data points equal to 0 when calculating the mean.